need a flow chart based on info Here’s a desсrіption of how the research was con

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need a flow chart based on info Here’s a desсrіption of how the research was conducted, followed by a suggestion for a flow chart to visually summarize the methodology:
Methodology
The research was conducted using a meta-analysis approach that compiled data from 126 independent nitrogen addition experiments. The following steps summarize the process:
1. Data Collection
• The authors identified 80 publications containing data from nitrogen addition studies across various biomes (e.g., forests, grasslands, tundra).
• Experiments were included if they measured aboveground net primary productivity (ANPP) after nitrogen fertilization in terrestrial ecosystems.
• Studies using greenhouse data, soil fertility gradients, or manipulations beyond nitrogen addition were excluded.
2. Experimental Design Selection
• Each experiment compared plant growth in fertilized (N-added) plots to control (unfertilized) plots.
• Only one measurement per study was used, selecting the highest rate of nitrogen addition and the earliest measurement after fertilization to minimize downstream effects.
3. Biomes Categorization
• Studies were grouped into biomes: temperate forests, tropical forests, temperate grasslands, tropical grasslands, tundra, wetlands, and deserts.
4. Climate and Geographical Data Collection
• Geographical coordinates, mean annual temperature (MAT), and mean annual precipitation (MAP) were retrieved for each study site using climate databases.
5. Calculation of Response Ratio
• A response ratio (R) was calculated for each experiment:

• This ratio indicates the degree of nitrogen limitation (R ˃ 1 reflects increased productivity due to nitrogen).
Proposed Flow Chart
The flow chart below summarizes the methodology visually:
1. Study Identification
• Search for relevant studies in the literature (80 publications, 126 experiments).
2. Criteria for Inclusion
• Must measure ANPP.
• Exclude greenhouse studies and non-nitrogen-specific treatments.
3. Categorization
• Group studies by biome and collect geographic and climate data.
4. Data Processing
• Extract ANPP data for fertilized and control plots.
• Calculate response ratio (R).
5. Analysis
• Analyze trends in nitrogen limitation across biomes and climatic/geographical factors.

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